Liriodendron Tulipifera L. EST-SSR Marker Development For Genetic Linkage Map Construction

Kayla Owens

School Name

South Carolina Governor's School for Science and Mathematics

Grade Level

12th Grade

Presentation Topic

Botany

Presentation Type

Mentored

Mentor

Mentor: Haiying Liang, Department of Genetics and Biochemistry, Clemson University

Written Paper Award

2nd Place

Abstract

Liriodendron tulipifera L., commonly known as yellow poplar, is a fast-growing hardwood tree species with great economic value, commonly used in the furniture and honey industries. Genomic resources, such as EST databases and BAC libraries, have been developed for this species. However, at this time, there is no framework genetic linkage map of L. tulipifera. Such a map would show the positions of genetic markers relative to one another on a chromosome. It will also allow the recombination frequency to be calculated, which would show the tendency of given genes to separate during meiosis. A framework genetic linkage map is necessary for improved molecular breeding, quantitative trait locus (QTL) mapping, and to provide a base for sequencing the Liriodendron genome in the future. In this project, available EST databases were mined for putative polymorphic sequence repeat (SSR) markers in order to develop 190 informative EST SSRs. The DNA used during this project was extracted from control-pollinated Liriodendron tulipifera L. plant matter via the CTAB method. Using this DNA, SSR markers were amplified via PCR. The successfully amplified PCRs will undergo fragment analysis in order to determine whether or not a given marker is informative, with distinct allele size differences among individuals. The markers found to be informative will be used to construct the first framework genetic linkage map of L. tulipifera with 300 full-sib seedlings

Start Date

4-11-2015 10:45 AM

End Date

4-11-2015 11:00 AM

COinS
 
Apr 11th, 10:45 AM Apr 11th, 11:00 AM

Liriodendron Tulipifera L. EST-SSR Marker Development For Genetic Linkage Map Construction

Liriodendron tulipifera L., commonly known as yellow poplar, is a fast-growing hardwood tree species with great economic value, commonly used in the furniture and honey industries. Genomic resources, such as EST databases and BAC libraries, have been developed for this species. However, at this time, there is no framework genetic linkage map of L. tulipifera. Such a map would show the positions of genetic markers relative to one another on a chromosome. It will also allow the recombination frequency to be calculated, which would show the tendency of given genes to separate during meiosis. A framework genetic linkage map is necessary for improved molecular breeding, quantitative trait locus (QTL) mapping, and to provide a base for sequencing the Liriodendron genome in the future. In this project, available EST databases were mined for putative polymorphic sequence repeat (SSR) markers in order to develop 190 informative EST SSRs. The DNA used during this project was extracted from control-pollinated Liriodendron tulipifera L. plant matter via the CTAB method. Using this DNA, SSR markers were amplified via PCR. The successfully amplified PCRs will undergo fragment analysis in order to determine whether or not a given marker is informative, with distinct allele size differences among individuals. The markers found to be informative will be used to construct the first framework genetic linkage map of L. tulipifera with 300 full-sib seedlings