Multiple PDZ Domain Protein Inhibits Angiogenic Sprouting In Human Umbilical Arterial Endothelial Cells (HUAEC)
School Name
South Carolina Governor's School for Science and Mathematics
Grade Level
12th Grade
Presentation Topic
Cell and Molecular Biology
Presentation Type
Mentored
Abstract
Like any human tissue, cancerous tissues in tumors require a constant source of oxygen, which is provided by blood vessels. To allow for tumor growth, angiogenesis, or the development of new blood vessel lumen from pre-existing lumen, must occur. A major component of angiogenesis is cell differentiation, and current scientific understanding suggests that Delta-Notch signaling plays an important role in this process. It also suggests that Multiple PDZ domain protein (MPDZ), a scaffolding protein found in endothelial cells, may influence this pathway. Previous research has found that in Human Umbilical Venous Endothelial Cells (HUVEC cells), knockdown of MPDZ results in lower angiogenic activity due to an increase in Delta-Notch signaling. The aim of this research is to determine whether lower levels of angiogenesis occurs in Human Umbilical Arterial Endothelial Cells (HUAEC cells) as well. It was tested through measuring the expression of several Notch target genes (Hey1, Hey2, NRARP, and EphrinB2) using RT:PCRs and through the production of spheroids of HUAEC cells which were induced to sprout under the presence/absence of VEGF and MPDZ control/knockdown. We found that MPDZ knockdown resulted in lower expression of the genes and lower levels of angiogenic sprouting compared to control groups, confirming that MPDZ inhibits angiogenesis in HUAEC cells.
Recommended Citation
Brown, Tyler, "Multiple PDZ Domain Protein Inhibits Angiogenic Sprouting In Human Umbilical Arterial Endothelial Cells (HUAEC)" (2015). South Carolina Junior Academy of Science. 78.
https://scholarexchange.furman.edu/scjas/2015/all/78
Start Date
4-11-2015 10:45 AM
End Date
4-11-2015 11:00 AM
Multiple PDZ Domain Protein Inhibits Angiogenic Sprouting In Human Umbilical Arterial Endothelial Cells (HUAEC)
Like any human tissue, cancerous tissues in tumors require a constant source of oxygen, which is provided by blood vessels. To allow for tumor growth, angiogenesis, or the development of new blood vessel lumen from pre-existing lumen, must occur. A major component of angiogenesis is cell differentiation, and current scientific understanding suggests that Delta-Notch signaling plays an important role in this process. It also suggests that Multiple PDZ domain protein (MPDZ), a scaffolding protein found in endothelial cells, may influence this pathway. Previous research has found that in Human Umbilical Venous Endothelial Cells (HUVEC cells), knockdown of MPDZ results in lower angiogenic activity due to an increase in Delta-Notch signaling. The aim of this research is to determine whether lower levels of angiogenesis occurs in Human Umbilical Arterial Endothelial Cells (HUAEC cells) as well. It was tested through measuring the expression of several Notch target genes (Hey1, Hey2, NRARP, and EphrinB2) using RT:PCRs and through the production of spheroids of HUAEC cells which were induced to sprout under the presence/absence of VEGF and MPDZ control/knockdown. We found that MPDZ knockdown resulted in lower expression of the genes and lower levels of angiogenic sprouting compared to control groups, confirming that MPDZ inhibits angiogenesis in HUAEC cells.
Mentor
Mentor: Fabian Tetzlaff, Vascular Signaling and Cancer, German Cancer Research Center (DKFZ)