The Effect of Linum usitatissimum Oil, EGCG and Punica granatum Extract on the Lifespan of Caenorhabditis elegans

Bhavani Tuppale

School Name

Spring Valley High School

Grade Level

10th Grade

Presentation Topic

Microbiology

Presentation Type

Non-Mentored

Abstract

Age-related research done on natural alternative medicine has helped in the contribution of the study of human lifespan. Aging research, due to studies on the aging of the global population, have recently been in urgent need of improvement in longevity for older generations. Research studies done today have focused on the use of natural alternatives to extend longevity of seniors (Williamson, A. T., Fletcher, P. C., & Dawson, K. A., 2003). This research was aimed to determine what extracts/oils presented the most noticeable increase in lifespan for Caenorhabditis elegans. It was hypothesized that if C.elegans are exposed to extracts/oils such as Linum usitatissimum oil, Epigallocatechin gallate and Punica granatum seed extract, then the use of EGCG would exhibit an increase in C.elegan lifespan. The results of the experiment, however, exhibited a significant increase in C.elegans in the oil Linum usitatissimum. This failed to support the original hypothesis since the data exhibits a greater interaction between the factors for Linum usitatissimum oil than in EGCG. A two-way ANOVA test displayed differences in the means of the counts between the extracts/oils used for the experimentation. In the results, Linum usitatissimum oil exhibits the highest count of C.elegans in comparison to the other two extracts and control. For the set of C.elegans in the stages of embryos to L3, there was a 77.11% coefficient of determination as well as a coefficient of determination of 83.39% for C.elegans in the stages of dauer to adult.

Location

Furman Hall 126

Start Date

3-28-2020 11:00 AM

Presentation Format

Oral and Written

Group Project

No

COinS
 
Mar 28th, 11:00 AM

The Effect of Linum usitatissimum Oil, EGCG and Punica granatum Extract on the Lifespan of Caenorhabditis elegans

Furman Hall 126

Age-related research done on natural alternative medicine has helped in the contribution of the study of human lifespan. Aging research, due to studies on the aging of the global population, have recently been in urgent need of improvement in longevity for older generations. Research studies done today have focused on the use of natural alternatives to extend longevity of seniors (Williamson, A. T., Fletcher, P. C., & Dawson, K. A., 2003). This research was aimed to determine what extracts/oils presented the most noticeable increase in lifespan for Caenorhabditis elegans. It was hypothesized that if C.elegans are exposed to extracts/oils such as Linum usitatissimum oil, Epigallocatechin gallate and Punica granatum seed extract, then the use of EGCG would exhibit an increase in C.elegan lifespan. The results of the experiment, however, exhibited a significant increase in C.elegans in the oil Linum usitatissimum. This failed to support the original hypothesis since the data exhibits a greater interaction between the factors for Linum usitatissimum oil than in EGCG. A two-way ANOVA test displayed differences in the means of the counts between the extracts/oils used for the experimentation. In the results, Linum usitatissimum oil exhibits the highest count of C.elegans in comparison to the other two extracts and control. For the set of C.elegans in the stages of embryos to L3, there was a 77.11% coefficient of determination as well as a coefficient of determination of 83.39% for C.elegans in the stages of dauer to adult.