The Synergistic Effects of Silibinin and Resveratrol on Pollen-Inflamed Mice Endothelial Cells

School Name

Spring Valley High School

Grade Level

11th Grade

Presentation Topic

Microbiology

Presentation Type

Non-Mentored

Abstract

Pollen is a fine powdery substance that gets released in the spring season and can negatively impact people due to its small size. When inhaled, the pollen can cause inflammation in vital airways, causing asthma and constricting breath. However, this can be hindered using natural means through Silibinin and Resveratrol, both of which contain healing compounds that can mitigate inflammation. Synergistic effects of two elements could also be used to increase the effectiveness of healing. Thus, the purpose of this study is to examine the synergistic effects of Silibinin and Resveratrol on pollen-inflamed mice endothelial cells. This was done to aid in closing the gap regarding whether plant polyphenols can be used to treat severe allergies that persist. Combination and mono-therapies of the polyphenols were used on the inflammation rates of mouse endothelial cells caused by the pollen. The study used two MTT Assays: one to assess the dose of pollen that caused 50% of cells to perish, and another to assess the effectiveness of the plant polyphenols on the inflamed cells. Finally, a microscopic study was performed to determine the mode of inhibition of cell death following drug treatment in inflamed cells. The results show that 250 µg/mL pollen can induce 50% cell death (IC50), and the combination treatment of Silibinin and Resveratrol most effectively inhibits the pollen-induced inflammation synergistically by preventing necrosis in endothelial cells. Collectively, the study shows that combination therapy with Silibinin and Resveratrol can be a potential therapeutic strategy against pollen-induced inflammatory diseases.

Location

Furman Hall 111

Start Date

3-28-2026 9:45 AM

Presentation Format

Oral and Written

Group Project

No

COinS
 
Mar 28th, 9:45 AM

The Synergistic Effects of Silibinin and Resveratrol on Pollen-Inflamed Mice Endothelial Cells

Furman Hall 111

Pollen is a fine powdery substance that gets released in the spring season and can negatively impact people due to its small size. When inhaled, the pollen can cause inflammation in vital airways, causing asthma and constricting breath. However, this can be hindered using natural means through Silibinin and Resveratrol, both of which contain healing compounds that can mitigate inflammation. Synergistic effects of two elements could also be used to increase the effectiveness of healing. Thus, the purpose of this study is to examine the synergistic effects of Silibinin and Resveratrol on pollen-inflamed mice endothelial cells. This was done to aid in closing the gap regarding whether plant polyphenols can be used to treat severe allergies that persist. Combination and mono-therapies of the polyphenols were used on the inflammation rates of mouse endothelial cells caused by the pollen. The study used two MTT Assays: one to assess the dose of pollen that caused 50% of cells to perish, and another to assess the effectiveness of the plant polyphenols on the inflamed cells. Finally, a microscopic study was performed to determine the mode of inhibition of cell death following drug treatment in inflamed cells. The results show that 250 µg/mL pollen can induce 50% cell death (IC50), and the combination treatment of Silibinin and Resveratrol most effectively inhibits the pollen-induced inflammation synergistically by preventing necrosis in endothelial cells. Collectively, the study shows that combination therapy with Silibinin and Resveratrol can be a potential therapeutic strategy against pollen-induced inflammatory diseases.